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China-CIMMYT collaboration enhances wheat improvement in China

Zhonghu HE, Xianchun XIA, Yong ZHANG, Yan ZHANG, Yonggui XIAO, Xinmin CHEN, Simin LI, Yuanfeng HAO, Awais RASHEED, Zhiyong XIN, Qiaosheng ZHUANG, Ennian YANG, Zheru FAN, Jun YAN, Ravi SINGH, Hans-Joachim BRAUN

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 3,   Pages 233-239 doi: 10.15302/J-FASE-2019254

Abstract: Genomic approaches have been used to develop and validate gene-specific markers, leading to the establishmentof a KASP platform, and seven cultivars have been released through application of molecular marker technology

Keywords： adult-plant resistance     bread wheat     breeding     gene-specific marker     germplasm exchange     processing quality    

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Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

Frontiers of Medicine 2007, Volume 1, Issue 3,   Pages 253-257 doi: 10.1007/s11684-007-0048-9

Abstract: construct the plasmid that could direct the synthesis of siRNA-like transcripts and thus mediate strong and specificrepression of human heat shock protein 90β (Hsp90β) gene expression and to compare the transfectionThree 64 nt oligos corresponding to different regions of the target gene were chemically synthesized

Keywords： HindIII     transfection efficiency     detection     enhanced     different    

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Erratum to “Targeted Genotyping of a Whole-Gene Repertoire by an Ultrahigh-Multiplex and Flexible HD-Marker Erratum

Pingping Liu,Jia Lv,Cen Ma,Tianqi Zhang,Xiaowen Huang,Zhihui Yang,Lingling Zhang,Jingjie Hu,Shi Wang,Zhenmin Bao,

Engineering 2022, Volume 18, Issue 11,   Pages 259-259 doi: 10.1016/j.eng.2022.09.002

Keywords： online     THE AUTHORS.Published     repertoire     behalf     Engineering     September     genotyping     Academy     HD-marker approach    

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Construction of 6HRE-GFAP-Baxα system specific for glioma gene therapy

TIAN Yongji, LI Guilin, GAO Jun, WANG Renzhi, KONG Yanguo, ZHANG Zhenxing, LI Shifang, TIAN Shiqiang, DOU Wanchen, ZHANG Bo

Frontiers of Medicine 2007, Volume 1, Issue 1,   Pages 49-53 doi: 10.1007/s11684-007-0010-x

Abstract: The aim of this paper is to construct a specific and high-performance gene therapy system for glioma.constructed a combined promoter 6HRE-GFAP (Hypoxia Responsive Element, Glial Fibrillary Acidic Protein) by genein glioma and tested its efficacy and specificity in cultured cells, and then constructed GFAP-Baxα geneIt was specific and effective for glioma gene therapy.

Keywords： GFAP-Baxα     BT325     Fibrillary     apoptotic     specificity    

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Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B

Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen

Frontiers of Medicine 2015, Volume 9, Issue 1,   Pages 90-99 doi: 10.1007/s11684-015-0390-2

Abstract:

Gene therapy provides a potential cure for hemophilia B, and significant progress has been achievedin liver-directed gene transfer mediated by adeno-associated viral vectors.vectors and hFIX expression cassette may positively contribute to the ultimate success of hemophilia B geneIn addition, the regulatory elements that control the FIX gene expression in these cassetteswere screened for liver-specific effectiveness.

Keywords： factor IX     hemophilia B     liver-specific regulatory elements     hydrodynamic gene transfer    

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Transcriptome resources and genome-wide marker development for Japanese larch (

Wanfeng LI,Suying HAN,Liwang QI,Shougong ZHANG

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 1,   Pages 77-84 doi: 10.15302/J-FASE-2014010

Abstract: While the differential responses of trees to changes in climatic and environmental conditions have been demonstrated as they age, the underlying mechanisms and age control of tree growth and development are complex and poorly understood particularly at a molecular level. In this paper, we present a transcriptome analysis of , a deciduous conifer that is widely-grown in the northern hemisphere and of significant ecological and economic value. Using high-throughput RNA sequencing, we obtained about 26 million reads from the stems of 1-, 2-, 5-, 10-, 25- and 50-year-old trees. Combining these with the published Roche 454 sequencing reads and the expressed sequence tags (both mainly from embryogenic cell cultures), we assembled 26670549 reads into 146786 transcripts, of which we annotated 79182 to support investigations of the molecular basis of tree aging and adaption, somatic embryogenesis and wood formation. Using these sequences we also identified many single-nucleotide polymorphisms, simple sequence repeats, and insertion and deletion markers to assist breeding and genetic diversity studies of .

Keywords： Larix     transcriptome     age     wood formation     somatic embryogenesis     molecular marker    

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Validation of

Youfen Xu, Zong Li, Ruyin Liu, Hongxia Liang, Zhisheng Yu, Hongxun Zhang

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 4, doi: 10.1007/s11783-020-1246-9

Abstract: . • The pig-specific MST assays can contribute to managing the pig fecal pollution.We aimed to compare the performance of five previously reported pig-specific MST assays (PF, Pig-Bac1SYBR, Pig-Bac2SYBR, Pig-1-BacTaqMan, and Pig-2-BacTaqMan, which are based on Bacteroidales 16S rRNA geneOur results regarding the specificity of these pig-specific MST assays differ from those reported in

Keywords： Microbial source tracking     Pig fecal pollution     16S rRNA gene markers     Pig-specific Bacteroidales    

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Identification of molecular markers linked to rice bacterial blight resistance genes from

Jing WANG,Chen CHENG,Yanru ZHOU,Yong YANG,Qiong MEI,Junmin LI,Ye CHENG,Chengqi YAN,Jianping CHEN

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3,   Pages 260-265 doi: 10.15302/J-FASE-2015070

Abstract: The SSR marker RM128 on chromosome 1, the STS marker R03D159 on chromosome 3 and the STS marker R05D104

Keywords： Oryza meyeriana     bacterial blight resistance gene     genetic population     linkage molecular marker    

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Chinmedomics facilitated quality-marker discovery of Sijunzi decoction to treat spleen qi deficiency

Qiqi Zhao, Xin Gao, Guangli Yan, Aihua Zhang, Hui Sun, Ying Han, Wenxiu Li, Liang Liu, Xijun Wang

Frontiers of Medicine 2020, Volume 14, Issue 3,   Pages 335-356 doi: 10.1007/s11684-019-0705-9

Abstract: A total of 58 biomarkers were related to SQDS, and SJZD can adjust a large proportion of marker metabolitesisoglabrolide, and glycyrrhetnic acid as the q-markers of licorice; and 2-atractylenolide as the q-marker

Keywords： traditional Chinese medicine     Sijunzi decoction     spleen qi deficiency syndrome     Chinmedomics     quality-marker    

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Protein phosphatase magnesium-dependent 1δ is a novel tumor marker and target in hepatocellular carcinoma

Zhi Xu,Chunxiang Cao,Haiyan Xia,Shujing Shi,Lingzhi Hong,Xiaowei Wei,Dongying Gu,Jianmin Bian,Zijun Liu,Wenbin Huang,Yixin Zhang,Song He,Nikki Pui-Yue Lee,Jinfei Chen

Frontiers of Medicine 2016, Volume 10, Issue 1,   Pages 52-60 doi: 10.1007/s11684-016-0433-3

Abstract: Kaplan-Meier and Cox regression data indicated that PPM1D overexpression was an independent predictor of HCC-specific Overall, results suggest that PPM1D is a potential prognostic marker and therapeutic target

Keywords： PPM1D     hepatocellular carcinoma     prognosis     target therapy    

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Novel polymorphic EST-based microsatellite marker isolation and characterization from

Manosh Kumar BISWAS,Peng CHEN,Mohamed Hamdy AMAR,Xiuxin DENG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 1,   Pages 60-65 doi: 10.15302/J-FASE-2015048

Abstract: Novel simple sequence repeat (SSR) markers were developed to evaluate their utility for genetic diversity and breeding studies of and related species. A total of 108 primer pairs were characterized by PCR amplification experiments. Among these, 61 were polymorphic and transferable to other citrus species. The number of alleles per locus ranged from 2 to 6, with an average of 2.37 alleles per locus. The expected heterozygosity and observed heterozygosity ranged from 0 to 0.83 and 0 to 1.00, respectively. These novel polymorphic SSR markers will be useful for citrus cultivar identification and evaluation as well as breeding studies.

Keywords： microsatellite     polymorphism     Poncirus trifoliata    

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NES1/KLK10 and hNIS gene therapy enhanced iodine-131 internal radiation in PC3 proliferation

Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li

Frontiers of Medicine 2019, Volume 13, Issue 6,   Pages 646-657 doi: 10.1007/s11684-018-0643-y

Abstract: gene is thought to be a tumor-suppressor gene.Our previous study found that overexpression of gene in PC3 cell line could slow down the tumor proliferationThus, we supposed to have an “enhanced firepower” effect by combining overexpressed gene therapy andmicro-positron emission tomography/computed tomography (micro-PET/CT) imaging showed that the combined gene

Keywords： androgen-independent prostate cancer     normal epithelial cell-specific 1/kallikrein 10     sodium/iodide symporter    

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Study on Family Collection, Gene Mapping, Gene Identification and Gene Function of Human Genetic Diseases

Xia Jiahui

Strategic Study of CAE 2000, Volume 2, Issue 11,   Pages 1-11

Abstract:

In this paper, the study on family collection, gene mapping, gene identification and gene functionUsing G-banding technique a marker chromosome t (1; 3) (q44; p11) associated with nasopharyngeal cancerwas found in 1975 at first, and human TDF gene was mapped to chromosome Yp11. 32 in 1981.ldquo;Gene Family-Candidate Disease Gene Cloning”，and the paper was published in Nature Geneticswas also cloned from gene function study.

Keywords： genetic disease     gene mapping and cloning     gene familiy-candidate disease gene cloning     genome wide scan     gene function study    

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Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4,   Pages 663-675 doi: 10.1007/s11705-017-1623-5

Abstract: Small interfering RNA (siRNA) therapeutics hold great promise to treat a variety of diseases, as long as they can be delivered safely and effectively into cells. Dendrimers are appealing vectors for siRNA delivery by virtue of their well-defined molecular architecture and multivalent cooperativity. However, the clinical translation of RNA therapeutics mediated by dendrimer delivery is hampered by the lack of dendrimers that are of high quality to meet good manufacturing practice standard. In this context, we have developed small amphiphilic dendrimers that self-assemble into supramolecular structures, which mimic high-generation dendrimers synthesized with covalent construction, yet are easy to produce in large amount and superior quality. Indeed, the concept of supramolecular dendrimers has proved to be very promising, and has opened up a new avenue for dendrimer-mediated siRNA delivery. A series of self-assembling supramolecular dendrimers have consequently been established, some of them out-performing the currently available nonviral vectors in delivering siRNA to various cell types and , including human primary cells and stem cells. This short review presents a brief introduction to RNAi therapeutics, the obstacles to their delivery and the advantages of dendrimer delivery vectors as well as our bio-inspired structurally flexible dendrimers for siRNA delivery. We then highlight our efforts in creating self-assembling amphiphilic dendrimers to construct supramolecular dendrimer nanosystems for effective siRNA delivery as well as the related structural alterations to enhance delivery efficiency. The advent of self-assembling supramolecular dendrimer nanovectors holds great promise and heralds a new era of dendrimer-mediated delivery of RNA therapeutics in biomedical applications.

Keywords： gene therapy     RNAi therapeutics     dendrimer     nanovectors     gene silencing    

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Influence of sprinkler irrigation droplet diameter, application intensity and specific power on flower

Yisheng ZHANG, Delan ZHU

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 2,   Pages 165-171 doi: 10.15302/J-FASE-2017145

Abstract: The influences of different parameters such as droplet diameter, application intensity, specific powerEquations of application intensity, specific power with sprinkler irrigation time and flower injury ratioThe results indicated that specific power has a significant correlation with injury, and flower damagearea increased as the increasing of the value of specific power for the same irrigation time., the threshold of impinging damage of application intensity was 25.30, 5.01 and 1.64 mm·h and the specific

Keywords： application intensity     damage     floriculture     flowers     specific power     sprinkler irrigation    

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Title Author Date Type Operation

China-CIMMYT collaboration enhances wheat improvement in China

Zhonghu HE, Xianchun XIA, Yong ZHANG, Yan ZHANG, Yonggui XIAO, Xinmin CHEN, Simin LI, Yuanfeng HAO, Awais RASHEED, Zhiyong XIN, Qiaosheng ZHUANG, Ennian YANG, Zheru FAN, Jun YAN, Ravi SINGH, Hans-Joachim BRAUN

Journal Article

Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

Journal Article

Erratum to “Targeted Genotyping of a Whole-Gene Repertoire by an Ultrahigh-Multiplex and Flexible HD-Marker

Pingping Liu,Jia Lv,Cen Ma,Tianqi Zhang,Xiaowen Huang,Zhihui Yang,Lingling Zhang,Jingjie Hu,Shi Wang,Zhenmin Bao,

Journal Article

Construction of 6HRE-GFAP-Baxα system specific for glioma gene therapy

TIAN Yongji, LI Guilin, GAO Jun, WANG Renzhi, KONG Yanguo, ZHANG Zhenxing, LI Shifang, TIAN Shiqiang, DOU Wanchen, ZHANG Bo

Journal Article

Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B

Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen

Journal Article

Transcriptome resources and genome-wide marker development for Japanese larch (

Wanfeng LI,Suying HAN,Liwang QI,Shougong ZHANG

Journal Article

Validation of

Youfen Xu, Zong Li, Ruyin Liu, Hongxia Liang, Zhisheng Yu, Hongxun Zhang

Journal Article

Identification of molecular markers linked to rice bacterial blight resistance genes from

Jing WANG,Chen CHENG,Yanru ZHOU,Yong YANG,Qiong MEI,Junmin LI,Ye CHENG,Chengqi YAN,Jianping CHEN

Journal Article

Chinmedomics facilitated quality-marker discovery of Sijunzi decoction to treat spleen qi deficiency

Qiqi Zhao, Xin Gao, Guangli Yan, Aihua Zhang, Hui Sun, Ying Han, Wenxiu Li, Liang Liu, Xijun Wang

Journal Article

Protein phosphatase magnesium-dependent 1δ is a novel tumor marker and target in hepatocellular carcinoma

Zhi Xu,Chunxiang Cao,Haiyan Xia,Shujing Shi,Lingzhi Hong,Xiaowei Wei,Dongying Gu,Jianmin Bian,Zijun Liu,Wenbin Huang,Yixin Zhang,Song He,Nikki Pui-Yue Lee,Jinfei Chen

Journal Article

Novel polymorphic EST-based microsatellite marker isolation and characterization from

Manosh Kumar BISWAS,Peng CHEN,Mohamed Hamdy AMAR,Xiuxin DENG

Journal Article

NES1/KLK10 and hNIS gene therapy enhanced iodine-131 internal radiation in PC3 proliferation

Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li

Journal Article

Study on Family Collection, Gene Mapping, Gene Identification and Gene Function of Human Genetic Diseases

Xia Jiahui

Journal Article

Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

Journal Article

Influence of sprinkler irrigation droplet diameter, application intensity and specific power on flower

Yisheng ZHANG, Delan ZHU

Journal Article